Effects of Zoledronic Acid on Human Gingival Fibroblasts and Human Umbilical Vein Endothelial Cells

We evaluated the effects of zoledronic acid (ZOL) on human gingival fibroblasts (HGFs) and umbilical vein endothelial cells (HUVECs) associated with wound healing in oral soft tissues. HGFs HUVECs were divided into two groups: a culture media control group exposed to ZOL (50 μM). Cell proliferation was measured after 2, 4, 6, 8 days. The migration ability for each experiment using assay. apoptosis rate confirmed Culture supernatants collected from experimental vascular growth factor (VEGF) production enzyme-linked immunosorbent assay (ELISA). Further, expression level VEGF-A compared real-time quantitative polymerase chain reaction. abilities both be suppressed by addition ZOL, resulting apoptosis. ELISA revealed that quantity VEGF produced significantly higher than until 2 days ZOL. In HGFs, mRNA levels intracellular increased demonstrating VEGF. contrast, HUVECs, although endogenous considerably decreased supernatant, indicating possibility abnormalities autocrine functions or signal transduction exogenous These data suggest utility therapeutic approaches directed toward signaling improve medication-related osteonecrosis jaw soft-tissue healing.